СРАВНИТЕЛЬНЫЙ АНАЛИЗ МЕТОДОВ ИССЛЕДОВАНИЯ АКТИВНОСТИ MG2+-ЗАВИСИМОЙ NA+/K+-АКТИВИРУЕМОЙ АТФАЗЫ В ТЕНЯХ ЭРИТРОЦИТОВ

Polina Anatolevna Petrova


Аннотация


В настоящем обзоре рассматриваются методические причины широкого диапазона значений активности Mg2+-зависимой Na+/K+-активируемой АТФазы теней эритроцитов, которые описываются у различных авторов. Установлено, что получаемые исследователями различия в показаниях активности фермента обусловлены методическими особенностями, связанными со способом получения Na+/K+-АТФазы и измерением ее активности, такими как особенности выделения и хранения теней эритроцитов (режим центрифугирования, концентрация и состав лизирующего раствора, время и температура гемолиза и замораживания), а также особенностями методов количественного определения белка и неорганического фосфора. На основании проведенного анализа данных литературы мы полагаем, что для наиболее точного определения активности Na+/K+-АТФазы рекомендуется использовать лизирующий буферный раствор с применением хелатора и методы Фиске-Суббароу и Лоури для определения неорганического фосфора и количественного содержания белка соответственно.

Ключевые слова


сравнительный анализ; тени эритроцитов

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Литература


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DOI: https://doi.org/10.12731/wsd-2017-4-2-150-166

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(c) 2018 Polina Anatolevna Petrova

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