СРАВНИТЕЛЬНЫЙ АНАЛИЗ МЕТОДОВ ИССЛЕДОВАНИЯ АКТИВНОСТИ MG2+-ЗАВИСИМОЙ NA+/K+-АКТИВИРУЕМОЙ АТФАЗЫ В ТЕНЯХ ЭРИТРОЦИТОВ

Polina Anatolevna Petrova


Аннотация


В настоящем обзоре рассматриваются методические причины широкого диапазона значений активности Mg2+-зависимой Na+/K+-активируемой АТФазы теней эритроцитов, которые описываются у различных авторов. Установлено, что получаемые исследователями различия в показаниях активности фермента обусловлены методическими особенностями, связанными со способом получения Na+/K+-АТФазы и измерением ее активности, такими как особенности выделения и хранения теней эритроцитов (режим центрифугирования, концентрация и состав лизирующего раствора, время и температура гемолиза и замораживания), а также особенностями методов количественного определения белка и неорганического фосфора. На основании проведенного анализа данных литературы мы полагаем, что для наиболее точного определения активности Na+/K+-АТФазы рекомендуется использовать лизирующий буферный раствор с применением хелатора и методы Фиске-Суббароу и Лоури для определения неорганического фосфора и количественного содержания белка соответственно.

Ключевые слова


сравнительный анализ; тени эритроцитов

Полный текст:

PDF>PDF

Литература


Boldyrev A.A. Rol’ Na/K-nasosa v vozbudimykh tkanyakh [Function of Tissues Na/K-pump in Excitable]. Zhurnal Sibirskogo federal’nogo universiteta. Biologiya [Journal of Siberian Federal University. Biology], 2008, vol. 3, no. 1, pp. 206–225.

Mosyagin V.V. Vliyanie vozrasta i fiziologicheskogo sostoyaniya na aktivnost’ fermentnykh sistem kletok, tkaney i organov zhivotnykh [Influence of age and physiological state on the activity of cells enzyme systems, tissues and organs of animals]. Doctor’s thesis. Moscow, 2011, 261 p.

Aughey R.J., Murphy K.T., Clark S.A., Garnham A.P., Snow R.J., Cameron-Smith D., Hawley J.A., McKenna M.J. Muscle Na-K-ATPase activity and isoform adaptations to intense interval exercise and training in well-trained athletes. J. Appl. Physiol., 2007, vol. 103, no. 1, pp. 39–47.

Aughey R.J., Gore C.J., Hahn A.G., Garnham A.P., Clark S.A., Petersen A.C., Roberts A.D., McKenna M.J. Chronic intermittent hypoxia and incremental cycling exercise independently depress muscle in vitro maximal Na/K-ATPase activity in well-trained athletes. J. Appl. Physiol., 2005, vol. 98, no. 1, pp. 186–192.

Malfatti C.R., Burgos L.Т, Rieger A, Rudger C.L, Turmina J.A., Pereira R.A., Pavlak J.L., Silva L.A., Osiecki R. Decreased Erythrocyte Na/K-ATPase activity and increased plasma TBARS in prehypertensive patients. Scientific World Journal, 2012, 5 p.

Raccah D., Gallice P., Pouget J., Vague P. Hypothesis: low Na/K-ATPase activity in the red cell membrane, a potential marker of the predisposition to diabetic neuropathy. Diabete Metab., 1992, vol. 3, pp. 236–241.

Yakusheva I.A., Orlova L.I. Metod opredeleniya aktivnosti adenozintrifosfataz v gemolizatakh eritrotsitov krovi cheloveka [А method for determining the activity of adenosinetriphosphatase in hemolysates of human blood erythrocytes]. Laboratornoe delo [Laboratory work], 1970, no. 8., pp. 497–501.

Boldyrev A., Bulygina E., Carpenter D., Schoner W. Glutamate receptors communicate with Na/K-ATPase in rat cerebellum granule cells: demonstration of differences in the action of several metabotropic and ionotropic glutamate agonists on intracellular reactive oxygen species and the sodium pump. J. Molec. Neurochem., 2003, vol. 21, no. 3, pp. 213–222.

Kazenov A.M., Maslova M.N., Shalabodov A.D. Issledovanie aktivnosti Na/K-ATFazy v jeritrocitah mlekopitajushhih [Study of the Na/K-ATPase activity of mammalian erythrocytes]. Biohimija [Biochemistry], 1984, vol. 49, issue 7, pp. 1089–1095.

Lishko V.K., Malysheva M.K., Grevizirskaya T.I. Izuchenie vzaimodejstvija Na/K-ATFazy membran i tenej jeritrocitov s ouabainom [Investigation of interaction of Na/K-ATPase of the membranes and ghosts of erythrocytes with ouabain]. Biohimija [Biochemistry], 1974, vol. 39, no. 1, pp. 60–66.

Xu L.F., Zhou X.F., Hu K., Tang S., Luo Y.C., Lu W. Establishment of a rabbit model of chronic obstructive sleep apnea and application in cardiovascular consequences. Chin. Med. J. (Engl.), 2017, vol. 130, no. 4, pp. 452–459.

Tong Z., Yu F., Liu Z., Liang H. Influence of ShuJinHuoXue tablets on ischemia reperfusion injury of animals’ skeletal muscle. Molecules, 2012, vol. 17, no. 7, pp. 8494–8505.

Rojas M., Díaz P., León P., González A.A., González M., Barrientos V., Pestov N.B., Alzamora R., Michea L. Mineralocorticoids modulate the expression of the beta-3 subunit of the Na/K-ATPase in the renal collecting duct. Channels (Austin.), 2017.

Borovskaya M.K., Kuznetsova E.E., Gorokhova V.G., Koryakina L.B., Kuril’skaya T.E., Pivovarov Yu.I. Strukturno-funktsional’naya kharakteristika membrany eritrotsita i ee izmeneniya pri patologii raznogo geneza [Structural and functional characteristics of membrane`s erythrocyte and its change of pathologies of various genesis]. Byulleten’ VSNTs SO RAMN [Acta biomedical scientifica], 2010, no.3, issue 73, pp. 334–354.

Reinila M., MacDonald E., Salem N.Jr., Linnoila M., Trams E.G. Standardized method for the determination of human erythrocyte membrane adenosine triphosphatases. Anal. Biochem., 1982, vol. 124, issue 1, pp. 19–26.

Charalambous B.M., Mir M.A. An improved procedure for the preparation and measurement of Na/K-ATPase in human erythrocytes. Biochim. Biophys. Acta, 1982, vol. 691, no. 1, pp. 71–82.

Voeykov V.L, Vilenskaya N.D., Lukashev M.E., Gurevich V.V. Vydelenie membran retikulotsitov krys, soderzhashchikh reguliruemye guanilovymi nukleotidami adenilattsiklazu i β-adrenergicheskie retseptory [Isolation of rat reticulocyte membranes containing guanil nucleotide sensitive adenilate cyclase and β-adrenergic receptors]. Bioorganicheskaya khimiya [The Russian Journal of Bioorganic Chemistry]. 1982, vol. 8, no. 4, pp. 524–532.

Dodge J., Mitchell C., Hanahan D. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys., 1963, vol.100, no. 1, pp. 119–130.

Bilezikian J.P., Spiegel A.M., Brown E.M., Aurbach G.D. Identification and persistence of 6-adrenergic receptors during maturation of the rat reticulocyte. Mol. Pharmacol., 1977, vol. 13, no. 5, pp. 775–785.

Limbird L.E., Gill M., Stadel J.M. Hickey A.R., Lefkowit R.J. Loss of beta-adrenergic receptor-guanine nucleotide regulatory protein interactions accompanies decline in catecholamine responsiveness of adenylate cyclase in maturing rat erythrocytes. J. Biol. Chem., 1980, vol. 255, no. 5, pp. 1854–1861.

Medvedeva I.A. Dinamika i mekhanizm izmeneniya aktivnosti Na/K-ATFazy eritrotsitov krys v usloviyakh stressa [Dynamics and mechanism of changes in the activity of Na/K-ATPase in erythrocytes of rats under stress]. Extended abstract of Сandidate’s thesis. Rostov-na-Donu, 1993, 24 p.

Al-Rabeei M.A.M., Chalabov S.I., Astaeva M.D., Klichhanov N.K. Оsmoticheskaja rezistentnost’’ jeritrocitov krys i koncentracija tiolovyh grupp belkov ih membrany zavisjat ot dlitel’’nosti umerennoj gipotermii [Osmotic fragility of erythrocytes and plasma membrane thiol concentration is depends on the duration of mild hypothermia in rats]. Sovremennye problemy nauki i obrazovanija [Modern problems of science and education], 2015, no. 3, 539 p.

Kukushkin A.I. Issledovanie strukturno-funktsional’nykh kharakteristik membran eritrotsitov pri zamorazhivanii do umerenno nizkikh temperatur (-30 / -70 gradusov C) [Study of the structural-functional characteristics of the erythrocyte membranes after freezing to moderately low temperatures (from -30 to -70 degrees C)]. Extended abstract of Candidate’s thesis. Kharkov, 1992, 19 p.

Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem., 1976, vol.72, no. 1-2, pp. 248–254.

Sedmak J.J., Grossberg S.E. A rapid, sensitive and versatile assay for protein using Coomassie Brilliant Blue G-250. Anal. Biochem., 1977, vol. 79, pp. 544–552.

Lowry O., Rosebrough N., Farr A., Randall R. Protein measurment with the folin phenol reagent. J.Biol.Chem., 1951, vol.193, no. 1, pp. 265–270.

Olson B., Markwell J. Assays for determination of protein concentration. Curr. Protoc. Pharmacol., 2007, Appendix 3:3A.

Ponomareva C.A. Golovchenko V.V. Patova O.A, Vanchikova E.V. Ovodov Yu. Sravnitel’nyy analiz spektrofotometricheskikh metodik opredeleniya massovoy doli belka v obraztsakh pektinovykh polisakharidov [Comparative analysis of the spectrophotometric methods of the protein amount determination in the pectic polysaccharide samples]. Bioorganicheskaya khimiya [Russian Journal of Bioorganic Chemistry ], 2015, vol. 41, no. 2, pp. 154–161.

Kyrov D.N. Issledovanie moduliruyushchikh effektov gemolizata eritrotsitov na aktivnost’ Na/K-ATFazy [The study of the modulating effects of the hemolysate erythrocytes on activity Na/K-ATPase]. Extended abstract of Сandidate’s thesis. Tyumen, 2006, 20 р.

Pisareva L.N. Parfenova E.V. Vliyanie iskhodnogo sostoyaniya membrannogo preparata aktivnosti Na/K-ATFazy na vzaimodeystvie s gistonami.. [The influence of the initial state of the membrane preparation activity Na/K-ATPase to the interaction with histones]. Biofizika membrane [Biophysics of membranes], 1973, pp. 514–517.

Tashkin V.Yu. Konkurentnyy transport ionov v tsitoplazmaticheskom kanale dostupa Na/K-ATFazy [Competitive ion transport in the cytoplasmic access channel of the Na/K-ATPase]. Extended abstract of Candidate’s thesis. Moscow, 2014. 26 p.

Kazenov A.M. Maslova M.N. Osobennosti aktivatsii detergentami Na/K-adenozintrifosfatazy golovnogo mozga pozvonochnykh [Peculiaties of activation of Na/K-ATPase from the brain of vertebrates by detergentes]. Biohimija [Biochemistry], 1980, vol. 14, no. 5, pp. 430–435.

Maturu P., Vaddi D.R., Pannuru P., Nallanchakravarthula V. Alterations in erythrocyte membrane fluidity and Na/K-ATPase activity in chronic alcoholics. Mol. Cell. Biochem., 2010, vol. 39, no. 1–2, pp. 35–42.

Vasilenko T.F. Vliyanie poverkhnostno-aktivnykh veshchestv na adenozintrifosfataznuyu aktivnost’ i 180-obmennye svoystva Na/K-ATFazy [Surface-active substances influence on adenosinetriphosphatase activity and 180-exchange properties of the Na/K-ATPase ]. Сandidate›s thesis. Leningrad, 1980, 146 p.

Hanahan D.J., Ekholm J.E. The expression of optimum ATPase activities in human erythrocytes. A comparison of different lytic procedures. Arch. Biochem. Biophys., 1978, vol. 187, no. 1, pp. 170–179.

Baykov A.A., Evtushenko A.A., Avaeva S.M. A malachite green procedure for orthophosphate determination and its use in alkaline phosphatase-based enzyme immunoassay. Anal. Biochem, 1988, vol. 171, pp. 266–270.

Fiske C.H., Subbarow Y. The colorimetric determination of phosphorus. J.Biol.Chem., 1925, vol. 66, pp. 373–400.

Vladimirov G.E., Misheneva V.S. Opredelenie fosfornykh soedineniy v ochen’ malykh kolichestvakh nervnoy tkani [Determination of phosphorus compounds in very small amounts of nervous tissue]. Trudy instituta fiziologii imeni I.P. Pavlova [Proceedings of the Institute of physiology named I.P. Pavlov], 1956, vol. 5, pp. 416–424.

Chen P.S., Toribara T.Y., Warner H. Microdetermination of phosphorus. Anal. Chem., 1956, vol. 28, pp. 1756–1758.

Bolotov M.P. Karetnikov P.V. Fotokolorimetricheskoe opredelenie mineral’nogo fosfora [Photocolorimetric determination of the mineral phosphorus]. Laboratornoe delo [Laboratory work], 1965, no. 1, pp. 30–33.

Bartolommei G., Moncelli M.R., Tadini-Buoninsegni F. A method to measure hydrolytic activity of adenosinetriphosphatases (ATPases). PLoS One, 2013, vol. 8, no. 3.

Fedorova E.Yu., Maksimov V.I. Vliyanie ingibitora i ionov elektrolitov na aktivnost’ ATFaz moloka korov cherno-pestroy porody [Influence of electrolyte ions and inhibitor on the activity of black-spotted cow milk ATP]. Izvestiya Orenburgskogo gosudarstvennogo agrarnogo universiteta [News of the Orenburg State Agrarian University], 2013, no. 6, issue 44, pp. 231–232.

Nikolaev A.Ya. Biologicheskaya khimiya [Biological chemistry]. Moscow, Meditsinskoe informatsionnoe agentstvo, 2004, 566 p.

Vinogradov A.P. Analiticheskaya khimiya elementov – Fosfor [Analytical chemistry of elements – Phosphorus]. Moscow, Nauka, 1974, 219 p.

Petrun’kina A.M. Prakticheskaya biokhimiya [Practical biochemistry]. Leningrad, Biomedgiz, 1961, 298 p.

Kodama T., Fukui K., Kometani K. The initial phosphate burst in ATP hydrolysis by myosin and subfragment-1 as studied by a modified malachite green method for determination of inorganic phosphate. J. Biochem., 1986, vol. 99, no. 5, pp. 1465–1472.

Muszbek L., Szabó T., Fésüs L. A high sensitive method for the measurement of ATPase activity. Anal. Biochem., 1977, vol. 77, no. 1, pp. 286–288.

Queiroz-Claret C., Meunier J.C. Staining technique for phosphatases in polyacrylamide gels. Anal. Biochem., 1993, vol. 209, no. 2, pp. 228–231.

Geladopoulos T.P., Sotiroudis T.G., Evangelopoulos A.E. A malachite green colorimetric assay for protein phosphatase activity. Anal. Biochem., 1991, vol.192, no. 1, pp. 112–116.

Cen X., Huang Y., Wang R., Chen Z., Wu Z. Simultaneous assay of Ca-ATPase and Na/K-ATPase activities of osteoblast rat by malachite greencolorimetic method. Hua Xi Yi Ke Da Xue Xue Baо, 1998, vol. 29, no. 4, pp. 427–430.

Itaya K, Ui M. A new micromethod for the colorimetric determination of inorganic phosphate. Clin. Chim. Acta, 1966, vol.14, no. 3, pp. 361–366.

Sompong W., Cheng H., Adisakwattana S. Protective effects of ferulic acid on high glucose-induced protein glycation, lipid peroxidation, and membrane ion pump activity in human erythrocytes. PLoS One, 2015, vol. 10, no.6.

Lowry O.H., Roberts N.R., Leiner K.Y., Wu M.L., Farr A.L., Albers R.W. The Quantitative histochemistry of brain. Iii. Ammon’s horn. J. Biol. Chem., 1954, vol. 207, no.1, pp. 39–49.

Timin O.A., Kliment’eva T.K., Serebrov V.Yu., Zhavoronok T.V., Kuz’menko D.I., Udintsev S.N. Biokhimicheskie metody issledovaniya v kliniko-diagnosticheskikh laboratoriyakh: prakticheskoe posobie [Biochemical methods of research in clinical diagnostic laboratories: a practical guide]. Tomsk, STT, 2002, 244 p. http://biokhimija.ru/mineraly/fosfor (accessed July 1, 2017).

Kuttner T., Cohen H.R. Micro colorimetric studies. J. Biol. Chem., 1927, vol. 75, pp. 517–531.

Basova E.M., Ivanov V.M. Spektrofotometricheskoe opredelenie ortofosfat-ionov v plastovykh vodakh dlya provedeniya indikatornykh issledovaniy [Spectrophotometric determination of orthophosphate ions in stratal waters for indicator of research]. Vestnik. Moskovskogo universiteta. Seriya 2. Khimiya. [Department of Analytical Chemistry], vol. 53, no. 3, pp. 165–180.




DOI: https://doi.org/10.12731/wsd-2017-4-2-150-166

Ссылки

  • На текущий момент ссылки отсутствуют.




(c) 2018 Polina Anatolevna Petrova

Лицензия Creative Commons
Это произведение доступно по лицензии Creative Commons «Attribution-NonCommercial-NoDerivatives» («Атрибуция — Некоммерческое использование — Без производных произведений») 4.0 Всемирная.

ISSN 2072-0831 (print)

ISSN 2307-9428 (online)

Контент доступен под лицензией Creative Commons Attribution-NonCommercial-NoDerivs 4.0.

HotLog Яндекс цитирования